Remedios Perni holds a PhD in English Philology with European Mention (2014) and was awarded the Extraordinary Doctoral Prize (2016). She also holds bachelor's degrees in both English Philology and Art History from the University of Murcia. Her doctoral dissertation was interdisciplinary in scope, exploring the relationships between the works of William Shakespeare and visual culture.

Her research focuses primarily on politically engaged appropriations of Shakespeare, as well as on fostering collaboration between academics and activists. She is the Principal Investigator, together with Juan José Bermúdez de Castro (University of the Balearic Islands), of the Spanish Ministry of Science and Innovation-funded research project Shakespeare, Activism and the Arts in the 21st Century (2023–2026). Previously, she led the project Poetics of Activism: Literary and Cultural Intersectionalities in the Contemporary English-Speaking World (2019–2021), funded by the Regional Ministry of Education, Culture and Sport of the Valencian Community. She is a member of the THALIS research group and has also participated in the research project AWESOME, devoted to women travel writers.

Perni has published on Ophelia's role in the histories of madness, melancholy, and photography, on Shakespeare in the digital age, and on Shakespearean appropriations in the work of Angélica Liddell. In 2023, she edited and published Shakespeare's First Folio Revisited: Quadricentennial Essays, a collection of essays by leading scholars in Shakespeare studies. From 2020 to 2024, she served as co-editor of the Revista Alicantina de Estudios Ingleses (RAEI).

She is also a translator of critical theory. Her translations include works by Elaine Showalter, Mieke Bal, W. J. T. Mitchell, Michel Fried, and Tim Murray, among others. In 2022, she received the Translation Award of the Spanish Association for Anglo-American Studies (AEDEAN).

In her spare time, she enjoys painting.