Molecular Microbiology

General data

Field of study:

Experimental Sciences

Director:

MARTINEZ MOJICA, FRANCISCO JUAN

Website:

E-mail:

fmojica@ua.es

Telephone:

+34 965903400 x 9761

Telephone:

+34 96 590 2162

Annual reports

Annual report

Study of repeated sequences in prokaryotes
Caracterización de las secuencias repetidas CRISPR (Clustered regularly interspaced short palindromic repeats) de procariotas. Significado funcional y aplicaciones en tipificación molecular. Implicaciones evolutivas. Componentes de un sitema de inmunidad frente a elementos genéticos foráneos basado en las repeticiones CRISPR. Aplicaciones en biotecnología y clínica.

Services offered
Results
Available relevant infrastructure

Services offered

Growth and manipulation of microorganisms. Detection and identification of microorganisms by molecular and culture methods. Genetic modification of prokaryotes. Analysis of gene expression in prokaryotes. Microbiological analysis of food.

Results

Identification in prokaryotes of a new genetic barrier against transmissible elements (plasmids and viruses) Development of a method for molecular typing of prokaryotes based on PCR amplification of CRISPR elements. Induction in prokaryotes of immunity against virus infection. Identification of virus components to be used as antibacterials.

Available relevant infrastructure

Basic equipment for experimentation in microbiology and molecular biology.

Proyectos See search box

Project type:

Public

Competitive

European

From

RELEVANT CONTRIBUTIONS TO RESEARCH PROJECTS

Project title: Agente de innovación para potenciar la transferencia de la tecnología generada en la Universidad de Alicante en el campo de la Microbiología al entono empresarial de la Comunidad ValencianaING
Reference: AVI20 ANEXO II

Competitive: Yes

European: No

Public: Yes

Project sponsors: AGENCIA VALENCIANA DE LA INNOVACION
Starting date: 01/01/2020
Termination date: 31/12/2021
Project title: Detección y caracterización de nuevos sistemas CRISPR-CasING
Reference:

Competitive: Yes

European: No

Public: Yes

Project sponsors: GENERALITAT VALENCIANA
Starting date: 01/11/2017
Termination date: 31/10/2021
Project title: Bacteriophage lysins as alternatives to antimicrobial treatment - BLAATING
Reference:

Competitive: Yes

European: No

Public: Yes

Project sponsors: MINISTERIO DE ECONOMIA Y EMPRESA
Starting date: 01/04/2016
Termination date: 31/03/2019
Project title: Inmunización en bacterias: adaptación del sistema CRISPR-Cas-I-E de Escherichia coli y Salmonella entericaING
Reference:

Competitive: Yes

European: No

Public: Yes

Project sponsors: MINISTERIO DE ECONOMIA Y EMPRESA
Starting date: 01/01/2015
Termination date: 31/12/2019
Project title: Caracterización del sistema CRISPR/CAS-Y de Escherichia coliING
Reference:

Competitive: Yes

European: No

Public: Yes

Project sponsors: MINISTERIO DE ECONOMIA Y EMPRESA
Starting date: 01/01/2012
Termination date: 31/12/2014
Project title: Investigación del sistema CRISPR-Cas IV-BING
Reference: GC2023

Competitive: Yes

European: No

Public: Yes

Project sponsors: MINISTERIO DE CIENCIA, INNOVACION Y UNIVERSIDADES
Starting date: 01/09/2024
Termination date: 31/08/2027
Project title: (PERIODICA-FI) FONDO DE INVESTIGACIÓN IVA SI ING
Reference:

Competitive: Yes

European: No

Public: Yes

Project sponsors: UNIVERSIDAD DE ALICANTE
Starting date: 30/05/2024
Termination date: 31/12/2030
Project title: Biotecnología de Extremófilos (BIOTECEXTREM) - VIGROB-016 ING
Reference: UADIF 2023

Competitive: Yes

European: No

Public: Yes

Project sponsors: UNIVERSIDAD DE ALICANTE
Starting date: 01/01/2024
Termination date: 31/12/2024
Project title: Biotecnología de Extremófilos (BIOTECEXTREM) - VIGROB-016 ING
Reference: VIGROB 2023

Competitive: Yes

European: No

Public: Yes

Project sponsors: UNIVERSIDAD DE ALICANTE
Starting date: 01/01/2024
Termination date: 31/12/2024
Project title: Mantenimiento de la Innoagent para promover la transferencia de conocimiento y resultados de la UA en el área de la Microbiología a las empresas de la Comunidad Valenciana.ING
Reference: IVACE+i 2024 ANEXO II

Competitive: Yes

European: No

Public: Yes

Project sponsors: AGENCIA VALENCIANA DE LA INNOVACION
Starting date: 01/01/2024
Termination date: 31/12/2026

Publications See search box

Books

Book chapters

Articles

Conferences

From year

To year

RELEVANT CONTRIBUTIONS IN JOURNALS

Title: Identification of the EH CRISPR-Cas9 system on a metagenome and its application to genome engineering
Authors: Esquerra, B.; Baquedano, I.; Ruiz, Raúl; Fernández, Almudena; Montoliu, Lluís; MARTINEZ MOJICA, FRANCISCO JUAN
Journals Microbial Biotechnology
Volume:
Pages: -
Date: 2023
ISSN: 1751-7915
DOI: http://dx.doi.org/10.1111/1751-7915.14266
Title: Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins
Authors: Alonso-Lerma, B; Jabalera, Y; Samperio, S; Morin, M; Fernandez, A; Hille, LT; Silverstein, RA; Quesada-Ganuza, A; Reifs, A; Fernández-Peñalver, S; , Benitez, Y.; Soletto, L; Gavira, JA; Diaz, A; Vranken, W; Sanchez-Mejias, A; Güell, M; Mojica, FJM; Kleinstiver, BP; Moreno-Pelayo, MA; , Montoliu, L.; Perez-Jimenez, R
Journals Nature Microbiology (Online)
Volume: 8
Pages: 77 - 90
Date: 2023
ISSN: 2058-5276
DOI: http://dx.doi.org/10.1038/s41564-022-01265-y
Title: Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities
Authors: Salgado, Oscar; , Guajardo-Leiva, S; Moya-Beltrán, Ana; Barbosa, Carla; Ridley, Christina; Tamayo-Leiva, Javier; Quatrini, Raquel; Mojica, FJM; , Diez, Beatriz
Journals Frontiers in Microbiology
Volume: 13
Pages: -
Date: 2022
ISSN: 1664-302X
DOI: http://dx.doi.org/10.3389/fmicb.2022.1069452
Title: Digging into the lesser-known aspects of CRISPR biology
Authors: Marco Guzmán, N.; Esquerra, B.; Mojica, FJM
Journals International Microbiology
Volume: 24
Pages: 473 - 498
Date: 2021
ISSN: 1139-6709
DOI: http://dx.doi.org/10.1007/s10123-021-00208-7
Title: Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants
Authors: , Makarova, Kira S; , Wolf, Yuri I; , Iranzo, Jaime; , Shmakov, Sergey A; , Alkhnbashi, Omer S; , Brouns, Stan J J; , Charpentier, Emmanuelle; , Cheng, David; , Haft, Daniel H; , Horvath, Philippe; , Moineau, Sylvain; Mojica, FJM; Scott, David; Shiraz, Shah; Siksnys, Virginijus; Terns, Michael; Venclovas, ¿eslovas; White, Malcolm F; , Yakunin A.F.; Yan, Winston; Feng, Zhang; , Garrett R.A.; , Backofen R; , van der Oost J.; , Barrangou R.; , Koonin E.V.
Journals Nature Reviews Microbiology
Volume: 18
Pages: 67 - 83
Date: 2020
ISSN: 1740-1526
DOI: http://dx.doi.org/10.1038/s41579-019-0299-x
Title: Efecto del pH del medio y de la concentración de iones magnesio sobre la actividad in vitro de temafloxacina (A-62254)
Authors: , Muñoz, C.; CATALAN, V.; , Gimeno, C.; , García De Lomas, J.
Journals Revista sin ISSN
Volume:
Pages: -
Date:
ISSN: 0000-0000
Title: Sequence diversity of the internal transcribed spacer (ITS) among different serogroups of Legionella pneumophila isolates
Authors: , Pascual, L; , Pérez, S.; , Moreno, A.; , Amo, A.; , Apraiz, D.; CATALAN, V.
Journals Revista sin ISSN
Volume:
Pages: -
Date:
ISSN: 0000-0000
Title: Deepening the knowledge of universal stress proteins in Haloferax mediterranei. Appl Microbiol Biotechnol. 2024;108(1):124. doi:10.1007/s00253-023-12899-1
Authors: Sanchez-Matarredona, L. ; Zafrilla, B.; Rubio-Portillo, E.; Bonete, M.J.; Esclapez, J.
Journals Applied Microbiology and Biotechnology
Volume: 108
Pages: 124 -
Date: 2024
ISSN: 0175-7598
DOI: http://dx.doi.org/10.1007/s00253-023-12899-1
Title: Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization.
Authors: Sanchez-Matarredona, L. ; García-Bonete, M. J. ; Guío, Jorge; Camacho, M.; Fillat, María F.; Esclapez, J.; Bonete, M.J.
Journals International Journal of Biological Macromolecules
Volume: 260
Pages: 129541 -
Date: 2024
ISSN: 0141-8130
DOI: https://doi.org/10.1016/j.ijbiomac.2024.129541
Title: Identification of the EH CRISPR-Cas9 system on a metagenome and its application to genome engineering
Authors: Esquerra, B.; Baquedano, I.; Ruiz, Raúl; Fernández, Almudena; Montoliu, Lluís; Mojica, FJM
Journals Microbial Biotechnology
Volume: 16
Pages: 1505 - 1523
Date: 2023
ISSN: 1751-7915
DOI: http://dx.doi.org/10.1111/1751-7915.14266

RELEVANT PUBLISHED BOOKS

No data.

RELEVANT PUBLISHED BOOK CHAPTERS

Book chapter title: Discovery and Seminal Developments in the CRISPR field
Authors: Mojica, FJM; , Garrett, R.
Editorial: Springer Berlin / Heidelberg
Pages: 1 - 32
Date: 2013
ISBN:
Book title: CRISPR-Cas Systems: RNA-mediated adaptive immunity in Bacteria and Archaea

DOI: 10.1007/978-3-642-34657-6_1
Book chapter title: Response to Osmotic Stress in a Haloarchaeal Genome: a Role for General Stress Proteins and Global Regulatory Mechanisms
Authors: , Juez, G; , Fenosa, D.; , Gonzaga A; Soria, E.; Mojica, FJM
Editorial: ASM Press (American Society for Microbiology)
Pages: 232 - 240
Date: 2007
ISBN:
Book title: Physiology and Biochemistry of Extremophiles
Book chapter title: Antiarchaeal Antibiotics.
Authors: Mojica, FJM
Editorial: Research Singpost
Pages: 83 - 108
Date: 2003
ISBN:
Book title: New approach in the use of antibiotics.
Book chapter title: Genetic diversity in uropathogenic Escherichia coli as shown by RAPD (Random Amplified Polymorphic DNA), 16S-23S RDNA spacers and adhesin gene restriction patterns.
Authors: Rodríguez-Valera, F.; García-Martínez, J.; Zorraquino, A.; Martínez-Murcia, A.J.
Editorial:
Pages: 65 - 68
Date: 1997
ISBN:
Book title: Ecology of Pathogenic Bacteria, Molecular and Evolutionary Aspects.
Book chapter title: Fundamentos de la PCR en Microbiología
Authors: CATALAN, V.
Editorial: Unipath
Pages: 80 - 81
Date: 1993
ISBN:
Book title: Aplicaciones de la PCR al control microbiológico del agua
Book chapter title: INVES 10: Evaluación colaborativa de aprendizajes mediante e-rúbricas en Iniciación a la Investigación en Biología
Authors: Girela, J. L.; Bonet, A.; Sánchez Sánchez, A.; Casas, J.L.; De Juan, E.; García-Martínez, J.; Garmendia, I.; Giannetti, A.; Maldonado-Caro, R.
Editorial:
Pages: 1571 - 1595
Date:
ISBN:
Book title: Memorias del Programa de Redes-I3CE de calidad, innovación e investigación en docencia universitaria. Convocatoria 2020-21

RUA: http://hdl.handle.net/10045/120837
Book chapter title: INVES9: El trabajo colaborativo en el aprendizaje basado en proyectos en Iniciación a la Investigación en Biología
Authors: Bonet, A.; Sánchez Sánchez, A.; , Sánchez; Casas, J.L.; , J.L. Casas, J.L.; De Juan, E.; Garmendia, I.; Girela, J. L.; García-Martínez, J.; Maldonado-Caro, R.; , Maldonado, R.D.; Martínez-Espinosa, R.M.
Editorial: Instituto de Ciencias de la Educación (ICE) de la Universidad de Alicante
Pages: 549 - 556
Date: 2020
ISBN:
Book title: Memorias del Programa de Redes-I3CE de calidad, innovación e investigación en docencia universitaria.Convocatoria 2019-20.

RUA: http://hdl.handle.net/10045/110633
Book chapter title: INVES 8: Diagnóstico del trabajo grupal y mejora de las estrategias y metodologías docentes en Iniciación a la investigación en Biología
Authors: Girela, J. L.; Bonet, A.; Sánchez Sánchez, A.; Casas, J.L.; De Juan, E.; Garmendia, I.; García-Martínez, J.; Maldonado-Caro, R.; Pire, C.
Editorial:
Pages: 803 - 827
Date: 2019
ISBN:
Book title: Memòries del Programa de Xarxes-I3CE de qualitat, innovació i investigació en docència universitària. Convocatòria 2018-19

RUA: http://hdl.handle.net/10045/99929
Book chapter title: Prólogo
Authors: Mojica, FJM
Editorial: Next Door Publishers
Pages: 21 - 27
Date: 2019
ISBN:
Book title: Editando genes: recorta, pega y colorea. Las maravillosas herramientas CRISPR.
Book chapter title: INVES 7: Diagnóstico y mejora de las estrategias y metodologías docentes en Iniciación a la investigación en Biología
Authors: Girela, J. L.; Bonet, A.; Sánchez Sánchez, A.; Casas, J.L.; De Juan, E.; Garmendia, I.; García-Martínez, J.; Guerrero, JR; Maldonado-Caro, R.; Pire, C.; Valdecanto
Editorial:
Pages: 899 - 919
Date: 2018
ISBN:
Book title: Memorias del Programa de Redes-I3CE de calidad, innovación e investigación en docencia universitaria. Convocatoria 2017-18

RUA: http://hdl.handle.net/10045/89030

RELEVANT CONFERENCE PAPERS

Title: Characterization of novel Cas9 nucleases from the deep ocean
Authors: Cerón, Julián; Olaso, Alba; Sánchez, Pablo; Vicencio, Jeremy; Mingarro, Gerard; Rajkovski, Dejan; Colizzi, Francesco; Hernandes Coutinho, Felipe; Sanchez-Bolaños, Carlos; Moreno-Mateos, MIguel A.; Vazquez-Vilar, Marta; Orzáez, Diego; Sanchéz-Amat, Antonio; Mojica, FJM; , Silvia G. Acinas
Type of participation: Ponencia
Conference name: EMBO Workshop CRISPR-Cas. From Biology to therapeutic applications
Event type: Internacional
City of performance: Sevilla (SEVILLA) (ESPAÑA)
Date of performance: 07/11/2023
Title: CRISPR vs. CRISPR
Authors: Rosselli, R. ; Espinosa J.; Marco Guzmán, N.; Mojica, FJM
Type of participation: Ponencia invitada
Conference name: EMBO Workshop CRISPR-Cas. From Biology to therapeutic applications
Event type: Internacional
City of performance: Sevilla (SEVILLA) (ESPAÑA)
Date of performance: 07/11/2023
Title: Exploring the potential of CRISPR-Cas module transfers between bacteria lineages to overcome Anti-CRISPR inhibition
Authors: Rosselli, R. ; Marco Guzmán, N.; Espinosa J.; Mojica, FJM
Type of participation: Ponencia
Conference name:
Event type: Internacional
City of performance: (ESPAÑA)
Date of performance: 25/10/2023
Title: Optimización de la producción de ficoeritrobilina y ficocianobilina
Authors: Esclapez, J.; Sanchez-Matarredona, L. ; Zafrilla Requena, Guillermo; Bonete, M.J.; Zafrilla, B.
Type of participation: Póster
Conference name: 45º Congreso de la SEBBM
Event type: Nacional
City of performance: Zaragoza (ZARAGOZA) (ESPAÑA)
Date of performance: 05/09/2023
Title: Structural studies of the Lrp transcription factor regulator of the haloarchaeon Haloferax mediterranei.
Authors: García-Bonete, M. J. ; Sanchez-Matarredona, L. ; Camacho, M.; Esclapez, J.; Bonete, M.J.
Type of participation: Póster
Conference name: 45º Congreso de la SEBBM
Event type: Nacional
City of performance: Zaragoza (ZARAGOZA) (ESPAÑA)
Date of performance: 05/09/2023
Title: Biotechnological applications of CRISPR tools
Authors: Montoliu, Lluís; Fernández, Almudena; Perez-Jimenez, R; Mojica, FJM
Type of participation: Ponencia invitada
Conference name: XVIII Congreso de la Sociedad Española de Biotecnología, SEBiot 2023
Event type: Nacional
City of performance: Madrid (MADRID) (ESPAÑA)
Date of performance: 17/07/2023
Title: Horizontal transfer of adaptation and effector modules as a potential mechanism for overcoming CRISPR-Cas system inhibitions
Authors: Rosselli, R. ; Marco Guzmán, N.; Espinosa J.; Mojica, FJM
Type of participation: Póster
Conference name: CRISPR CONFERENCE 2023
Event type: Internacional
City of performance: Wu¿rzburg (ALEMANIA)
Date of performance: 27/06/2023
Title: Characterisation of Type IV CRISPR/Cas-carrying plasmids in Klebsiella spp.
Authors: Fernández-Favieres, Francisco Javier; Serna, Carlos; Dolcet-Negre, Marta M; Pulido-Vadillo, Mario; Montero, Natalia; , Riccardo Rosselli; Mojica, FJM; Gonzalez-Zorn, Bruno
Type of participation: Póster
Conference name: XIII Reunión del Grupo de Microbiología Molecular de la Sociedad Española de Microbiología
Event type: Nacional
City of performance: Granada (GRANADA) (ESPAÑA)
Date of performance: 07/09/2022
Title: Desarrollo de estrategias basadas en endolisinas para el control de patógenos vegetales
Authors: García-Martínez, J.
Type of participation: Ponencia invitada
Conference name: València BC: Simposi Internacional sobre Biocontrol en Agricultura Comunitat Valenciana
Event type: Internacional
City of performance: Valencia (VALENCIA/ VALÈNCIA) (ESPAÑA)
Date of performance: 04/07/2022
Title: The joy of CRISPR discovery
Authors: Mojica, FJM
Type of participation: Ponencia invitada
Conference name: IX International Symposium SRUK/CERU
Event type: Internacional
City of performance: Oxford (REINO UNIDO (Gran Bretaña e Irlanda del Norte))
Date of performance: 01/07/2022